Proteomics,Multiomics

Dataset Information

71

MRNA binding


ABSTRACT: RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings.

REANALYSED by: GPM11210041750

OTHER RELATED OMICS DATASETS IN: E-MTAB-869

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo sapiens  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Sophia Foehr   

PROVIDER: PXD002880 | Pride | 2015-09-11

REPOSITORIES: Pride

altmetric image

Publications


RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-bin  ...[more]

Similar Datasets

2016-03-31 | PXD003664 | Pride
2016-08-08 | PXD000883 | Pride
2016-07-11 | PXD003882 | Pride
2017-02-08 | PXD005601 | Pride
2016-10-21 | MSV000080265 | Massive
| GPM11210041750 | GPMDB
| GSE78237 | GEO
2018-08-29 | PXD008914 | Pride
2016-04-26 | PXD002992 | Pride
2018-12-17 | PXD009668 | Pride