Dataset Information

Comparative proteome of two citrus species differing in aluminum-tolerance in response to aluminum-toxicity revealed by iTRAQ

ABSTRACT: After long-term aluminum treatment, thereafter, we investigated leaves protein profiles of two citrus cultivars using isobaric tags for relative and absolute quantification (iTRAQ).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Citrus Maxima (pomelo) (citrus Grandis) Citrus Sinensis (sweet Orange) (citrus Aurantium Var. Sinensis)

TISSUE(S): Plant Cell, Leaf

DISEASE(S): Disease Free

SUBMITTER: Lin-Tong Yang

LAB HEAD: Lisong Chen

PROVIDER: PXD002916 | Pride | 2018-10-24

REPOSITORIES: Pride

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Publications

Aluminum Toxicity-Induced Alterations of Leaf Proteome in Two Citrus Species Differing in Aluminum Tolerance.

Li Huan H Yang Lin-Tong LT Qi Yi-Ping YP Guo Peng P Lu Yi-Bin YB Chen Li-Song LS

International journal of molecular sciences 20160721 7

Seedlings of aluminum-tolerant 'Xuegan' (Citrus sinensis) and Al-intolerant 'sour pummelo' (Citrus grandis) were fertigated for 18 weeks with nutrient solution containing 0 and 1.2 mM AlCl₃·6H₂O. Al toxicity-induced inhibition of photosynthesis and the decrease of total soluble protein only occurred in C. grandis leaves, demonstrating that C. sinensis had higher Al tolerance than C. grandis. Using isobaric tags for relative and absolute quantification (iTRAQ), we obtained more Al toxicity-respon ...[more]

PMID: 27455238

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Project description:The biological roles of ASRs (Abscisic acid, Stress, Ripening proteins) involved in certain crucial switches of plant development (grain and pollen germination, senescence, fruit ripening), and responses to environmental cues (drought, salinity, cold, nutrient depletion, fungus infection, heavy metal toxicity) remain still elusive. The functional duality of ASRs, as chaperones for direct protection of biological macromolecules, and as transcriptional regulators of gene expression, needs in-depth understanding of the underlying mechanisms. To further elucidate the biological functions of ASRs a quantitative proteomic analysis (iTRAQ) was performed comparing the nuclear proteins of grapevine embryogenic 41B cells: wild-type (WT) and genetically modified VvMSA-RNAi cells, silenced for grape ASR. Among all identified proteins, thirty-one were differentially expressed in WT and VvMSA-silenced cells and may be classified into five functional groups: metabolism, stress responses, ribosome biogenesis, transcription and chromatin architecture/remodeling. The impact of VvMSA silencing was also confirmed at the level of the genes encoding the differentially expressed nuclear proteins, and the revealed most pronounced effects at protein level suggest a more direct correlation to the putative biological function. Remarkably, many of the thirty-one differentially expressed proteins are supposedly involved in epigenetic events. To further corroborate this hypothesis immunoblot assay of Multiscreen type was performed using twenty-one antibodies to different post-translational modifications (PTMs) of histones H3 and H4 in both cell types, WT and VvMSA-RNAi. The subsequent ImageQuant analysis revealed marked semi-quantitative differences in most PTMs of grape histones H3 and H4, with sharp increase of H3K27ac, H3K36me3, H4K5ac and H4K12ac, as well as a strong decrease of H3K27me3 in VvMSA-RNAi cells. These findings provide new insights on a plausible role of VvMSA as an ASR protein involved in epigenetic mechanisms

Dataset Information

Comparative proteome of two citrus species differing in aluminum-tolerance in response to aluminum-toxicity revealed by iTRAQ

Publications

Aluminum Toxicity-Induced Alterations of Leaf Proteome in Two Citrus Species Differing in Aluminum Tolerance.

Similar Datasets

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