Dataset Information

Proteome analysis of testis from infertile protein C inhibitor deficient mice reveals predominant changes in serpin processing and prostaglandin metabolism

ABSTRACT: Serine protease inhibitors have therapeutic potential in a variety of pathogenic processes, ranging from thrombosis, altered immune response to liver cirrhosis. To investigate the physiological effects of protein C inhibitor (PCI, serpinA5) this gene was inactivated in a mouse model with the resulting phenotype of male infertility. In the actual report 2D-DIGE analysis was utilized to investigate the molecular mechanisms for PCI in male reproduction. Comparing the testes proteome of three PCI knockout-mice with the three wild-types- demonstrated similar pattern with the exception of a massive upregulation of prostaglandin reductase 1 (10-fold; P < 0.002) and complete shifts in the molecular weights of serpinA1C and serpinA3K. All these PCI-dependent proteome changes were immunologically verified. Unbiased proteome analysis indicated that the inactivation of serpinA5 strongly influenced both the protein species pattern of other A-clade serpins as well as the prostaglandin metabolism in the testes.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: Goran Mitulović

LAB HEAD: Maria Zellner

PROVIDER: PXD002944 | Pride | 2015-12-07

REPOSITORIES: Pride

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Publications

Stereotactic radiosurgery for spinal metastases from renal cell carcinoma.

Gerszten Peter C PC Burton Steven A SA Ozhasoglu Cihat C Vogel William J WJ Welch William C WC Baar Joseph J Friedland David M DM

Journal of neurosurgery. Spine 20051001 4

<h4>Object</h4>The role of stereotactic radiosurgery in treating renal cell carcinoma (RCC) metastases to the spine has previously been limited. In this study the authors evaluated the clinical outcome in patients with spinal RCC who underwent single-fraction radiosurgery.<h4>Methods</h4>Forty-eight patients with 60 RCC metastases to the spine (six cervical, 26 thoracic, 18 lumbar, and 10 sacral) were treated with a single-fraction radiosurgery technique and were followed for a period of 14 to 4 ...[more]

PMID: 16266070

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