Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)
TISSUE(S): Brain
SUBMITTER: Hongbo Guo
LAB HEAD: Avijit Chakrabartty
PROVIDER: PXD002959 | Pride | 2015-11-18
REPOSITORIES: Pride
eLife 20150929
Spatially targeted optical microproteomics (STOMP) is a novel proteomics technique for interrogating micron-scale regions of interest (ROIs) in mammalian tissue, with no requirement for genetic manipulation. Methanol or formalin-fixed specimens are stained with fluorescent dyes or antibodies to visualize ROIs, then soaked in solutions containing the photo-tag: 4-benzoylbenzyl-glycyl-hexahistidine. Confocal imaging along with two photon excitation are used to covalently couple photo-tags to all p ...[more]