Dataset Information

Interactome - RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage

ABSTRACT: AP-MS from non-crosslinked HeLa cells was carried out as described in the paper.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Matthias Groh

LAB HEAD: Natalia Gromak

PROVIDER: PXD002960 | Pride | 2018-05-16

REPOSITORIES: Pride

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RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage.

Cristini Agnese A Groh Matthias M Kristiansen Maiken S MS Gromak Natalia N

Cell reports 20180501 6

R-loops comprise an RNA/DNA hybrid and displaced single-stranded DNA. They play important biological roles and are implicated in pathology. Even so, proteins recognizing these structures are largely undefined. Using affinity purification with the S9.6 antibody coupled to mass spectrometry, we defined the RNA/DNA hybrid interactome in HeLa cells. This consists of known R-loop-associated factors SRSF1, FACT, and Top1, and yet uncharacterized interactors, including helicases, RNA processing, DNA re ...[more]

PMID: 29742442

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	Experiment1_IP.raw	Raw
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