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RACK1 interactome in drosophila S2 cells (FLAG-tag), using DDA and DIA (SWATH) approaches

ABSTRACT: Receptor for Activated C kinase 1 (RACK1) is a scaffold protein that has been found in association with several signaling complexes, and with the 40S subunit of the ribosome. Data presented here allowed a proteomic characterization of the interactome of RACK1 in drosophila S2 cells. We carried out Label-Free quantitation using both Data-Dependent (DDA) and Data-Independent Acquisition (DIA, SWATH). This study is a 2-factor experiment, where control samples (RACK1 overexpressed with a HA tag) were compared to IP samples (RACK1 overexpressed with a FLAG tag), in either mock or infected cells. Each of these 6 conditions were performed in biological triplicates.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture

SUBMITTER: Lauriane Kuhn

LAB HEAD: Philippe Hammann

PROVIDER: PXD002965 | Pride | 2017-05-29

REPOSITORIES: Pride

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Definition of a RACK1 Interaction Network in Drosophila melanogaster Using SWATH-MS.

Kuhn Lauriane L Majzoub Karim K Einhorn Evelyne E Chicher Johana J Pompon Julien J Imler Jean-Luc JL Hammann Philippe P Meignin Carine C

G3 (Bethesda, Md.) 20170705 7

Receptor for Activated protein C kinase 1 (RACK1) is a scaffold protein that has been found in association with several signaling complexes, and with the 40S subunit of the ribosome. Using the model organism Drosophila melanogaster, we recently showed that RACK1 is required at the ribosome for internal ribosome entry site (IRES)-mediated translation of viruses. Here, we report a proteomic characterization of the interactome of RACK1 in Drosophila S2 cells. We carried out Label-Free ...[more]

PMID: 28522639

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