Proteomics

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RACK1 interactome in drosophila S2 cells (FLAG-tag), using DDA and DIA (SWATH) approaches

ABSTRACT: Receptor for Activated C kinase 1 (RACK1) is a scaffold protein that has been found in association with several signaling complexes, and with the 40S subunit of the ribosome. Data presented here allowed a proteomic characterization of the interactome of RACK1 in drosophila S2 cells. We carried out Label-Free quantitation using both Data-Dependent (DDA) and Data-Independent Acquisition (DIA, SWATH). This study is a 2-factor experiment, where control samples (RACK1 overexpressed with a HA tag) were compared to IP samples (RACK1 overexpressed with a FLAG tag), in either mock or infected cells. Each of these 6 conditions were performed in biological triplicates.

INSTRUMENT(S):

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture

SUBMITTER: Lauriane Kuhn  

LAB HEAD: Philippe Hammann

PROVIDER: PXD002965 | Pride | 2017-05-29

REPOSITORIES: Pride

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Action DRS
2012_72_Ctrl_CrPV1_IDA.mgf Mgf
2012_72_Ctrl_CrPV2_b_IDA.mgf Mgf
2012_72_Ctrl_CrPV3_IDA.mgf Mgf
2012_72_Ctrl_NI1_IDA.mgf Mgf
2012_72_Ctrl_NI2_IDA.mgf Mgf
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Publications

Definition of a RACK1 Interaction Network in <i>Drosophila melanogaster</i> Using SWATH-MS.

Kuhn Lauriane L   Majzoub Karim K   Einhorn Evelyne E   Chicher Johana J   Pompon Julien J   Imler Jean-Luc JL   Hammann Philippe P   Meignin Carine C  

G3 (Bethesda, Md.) 20170705 7

Receptor for Activated protein C kinase 1 (RACK1) is a scaffold protein that has been found in association with several signaling complexes, and with the 40S subunit of the ribosome. Using the model organism <i>Drosophila melanogaster</i>, we recently showed that RACK1 is required at the ribosome for internal ribosome entry site (IRES)-mediated translation of viruses. Here, we report a proteomic characterization of the interactome of RACK1 in <i>Drosophila</i> S2 cells. We carried out Label-Free  ...[more]

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