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Lineage specific metabolic activity in haematopoiesis

ABSTRACT: Proteins were relatively quantified from the isolated mitochondria undergoing differentiation along two distinct lineages.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hematopoietic Stem Cell

SUBMITTER: Michael Walker

LAB HEAD: Anthony Whetton

PROVIDER: PXD002968 | Pride | 2017-09-07

REPOSITORIES: Pride

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Features of lineage-specific hematopoietic metabolism revealed by mitochondrial proteomics.

Billing Claudia C Walker Michael M Noack Nicole N Böhme Christian C Ceglarek Uta U Niederwieser Dietger D Whetton Anthony A Cross Michael M

Proteomics 20170726 15-16

Hematopoietic bone marrow is a regenerative tissue of high clinical relevance, yet relatively little is known about the metabolism of the stem and progenitor populations concerned. We have used a multipotent murine cell line to generate sufficient numbers of cells undergoing self-renewal, erythroid or myeloid differentiation to allow a proteomics analysis of enriched mitochondria. Stringent analysis identified 37 mitochondria-associated proteins changing on differentiation in this system. Those ...[more]

PMID: 28665046

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