Dataset Information

Mouse Liver S-Palmitoylation ZDHHC13 LC-MS/MS

ABSTRACT: Protein S-palmitoylation is a reversible post-translational modification which adding 16-carbon palmitate to specific cysteines and contributes to variable biological functions such as protein trafficking, scaffolding and signaling. ZDHHC13 is one of the members of DHHC-containing palmitoyl-acyltransferase (PAT) family of enzyme. The Zdhhc13 deficient mice show the most severe phenotype with amyloidosis, alopecia, and osteoporosis. Here we used quantitative proteomic approach, combining resin-assisted capture (RAC) method and mass spectrometry-based label free strategy to identify the candidate Zdhhc13 substrates from mouse liver tissue.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

DISEASE(S): Osteoporosis,Alopecia,Lipid Metabolism Disorder,Amyloidosis

SUBMITTER: Yi-Ju Chen

LAB HEAD: Yi-Ju Chen

PROVIDER: PXD003039 | Pride | 2017-09-29

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	130928-LiverPalmCys-allCombined.mgf	Mgf
	131218-Liverx3Palm-SH-XL-all.mgf	Mgf
	131218_liverK1HAPalmSH.mgf	Mgf
	131218_liverK1PalmSH.mgf	Mgf
	131218_liverK2HAPalmSH.mgf	Mgf

Items per page:

1 - 5 of 175

Publications

Role of S-Palmitoylation by ZDHHC13 in Mitochondrial function and Metabolism in Liver.

Shen Li-Fen LF Chen Yi-Ju YJ Liu Kai-Ming KM Haddad Amir N Saleem ANS Song I-Wen IW Roan Hsiao-Yuh HY Chen Li-Ying LY Yen Jeffrey J Y JJY Chen Yu-Ju YJ Wu Jer-Yuarn JY Chen Yuan-Tsong YT

Scientific reports 20170519 1

Palmitoyltransferase (PAT) catalyses protein S-palmitoylation which adds 16-carbon palmitate to specific cysteines and contributes to various biological functions. We previously reported that in mice, deficiency of Zdhhc13, a member of the PAT family, causes severe phenotypes including amyloidosis, alopecia, and osteoporosis. Here, we show that Zdhhc13 deficiency results in abnormal liver function, lipid abnormalities, and hypermetabolism. To elucidate the molecular mechanisms underlying these d ...[more]

PMID: 28526873

Similar Datasets

Project description:The development of transcriptomic tools has allowed exhaustive description of stress responses. These responses always superimpose a general response associated to growth rate decrease and a specific one corresponding to the stress. The exclusive growth rate response can be achieved through chemostat cultivation, enabling all parameters to remain constant except the growth rate. We analysed metabolic and transcriptomic responses of Lactococcus lactis in continuous cultures at different growth rates ranging from 0.09 to 0.47 h-1. Growth rate was conditioned by isoleucine supply. Although the metabolism was constant and homolactic, a widespread transcriptomic response involving 30 % of the genome was observed. The expression of genes encoding physiological functions associated with biogenesis increased with growth rate (transcription, translation, fatty acid and phospholipids metabolism). Many phages, prophages and transposons related genes were down regulated by growth rate suggesting genome plasticity to be involved in the adaptation to slow growth. The growth rate response was compared to carbon and amino-acid starvation transcriptomic responses, revealing constant and significant involvement of growth rate regulations in these two stressful conditions (overlap 26%). Although stringent response mechanism is considered as the one governing growth deceleration in bacteria, the rigorous comparison of the two transcriptomic responses clearly indicated the mechanisms are distinct. Moreover it was established that genes positively regulated by growth rate are preferentially located in the vicinity of replication origin while those negatively regulated are mainly encountered at the opposite. This result demonstrates the often neglected relationship between genes expression and their location on chromosome. Keywords: growth rate impact, continuous cultures Continuous cultivation of Lactococcus lactis IL1403 were carried out on a chemically defined medium and under controlled conditions (30 Â°C, pH 6.6, nitrogen atmosphere). Cell samples were harvested at steady state. Total RNA was extracted from these samples and radiolabelled cDNA were prepared and hybridized on nylon arrays. 1948 amplicons specific of Lactococcus lactis IL1403 genes were spotted twice on the array. Samples corresponding to various growth rates were analyzed simultaneously and 3 independent repetitions were performed.