HYPE mediated AMPylation site profiling with YnATP in human lysates

ABSTRACT: We describe here the first example of global chemoproteomic screening for HYPE mediated AMPylation sites of human proteins. Catalytically active mutant HYPE E234G was applied in combination with an alkynylated ATP and an alkynyl-biotinylated side ID reagent for capture of chemoenzymatically tagged proteins. AMPylation sites were detected by shotgun proteomics performed on a Q-Exactive instrument.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Remigiusz Serwa  

LAB HEAD: Edward Tate

PROVIDER: PXD003053 | Pride | 2015-12-02

REPOSITORIES: Pride