Dataset Information


Top-down characterization of lunasin in a commercial soybean-derived product

ABSTRACT: The presence of lunasin was investigated in two soybean products, raw soy seeds and a commercial soybean beverage powder. Lunasin from the commercial soybean derivative was purified by reverse phase high pressure liquid chromatography and bystrong anion exchange solid phase extraction. Lunasin was further characterized by Top-down mass spectrometry (MS). The top-down characterization of lunasin unraveled a wide range of post-translationally modified proteoforms.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Glycine max  


DISEASE(S): Not Available

SUBMITTER: Aida Serra  

LAB HEAD: Siu Kwan Sze

PROVIDER: PXD003064 | Pride | 2016-05-24


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Commercial processed soy-based food product contains glycated and glycoxidated lunasin proteoforms.

Serra Aida A   Gallart-Palau Xavier X   See-Toh Rachel Su-En RS   Hemu Xinya X   Tam James P JP   Sze Siu Kwan SK  

Scientific reports 20160518

Nutraceuticals have been proposed to exert positive effects on human health and confer protection against many chronic diseases. A major bioactive component of soy-based foods is lunasin peptide, which has potential to exert a major impact on the health of human consumers worldwide, but the biochemical features of dietary lunasin still remain poorly characterized. In this study, lunasin was purified from a soy-based food product via strong anion exchange solid phase extraction and then subjected  ...[more]

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