Absolute quantification of budding yeast proteome with PCS method

ABSTRACT: Absolute quantification of proteome is one of the most important tasks in proteomic research. The aim in this analysis is providing proof-of-principle of our originally developed strategy for absolute quantification of multiple proteins present at various concentrations in a cell, using stable isotope-labeled peptide concatenated standard (PCS). This study also provides insights into structures of regulation of metabolic pathway and into biological mechanisms for regulation of expressed protein abundance.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Keiji Kito  

LAB HEAD: Keiji Kito

PROVIDER: PXD003084 | Pride | 2016-10-06

REPOSITORIES: Pride