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Yeast cell wall glycoproteomic SWATH-MS

ABSTRACT: Identification and relative quantification of cell wall proteins and glycan micro- and macroheterogeneity, in various mutants in N-glycan biosynthesis, measured by SWATH-MS

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Benjamin Schulz

LAB HEAD: Benjamin Luke Schulz

PROVIDER: PXD003091 | Pride | 2016-04-21

REPOSITORIES: Pride

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SWATH-MS Glycoproteomics Reveals Consequences of Defects in the Glycosylation Machinery.

Zacchi Lucia F LF Schulz Benjamin L BL

Molecular & cellular proteomics : MCP 20160419 7

Glycan macro- and microheterogeneity have profound impacts on protein folding and function. This heterogeneity can be regulated by physiological or environmental factors. However, unregulated heterogeneity can lead to disease, and mutations in the glycosylation process cause a growing number of Congenital Disorders of Glycosylation. We systematically studied how mutations in the N-glycosylation pathway lead to defects in mature proteins using all viable Saccharomyces cerevisiae strains with dele ...[more]

PMID: 27094473

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