Proteomics

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Identification of the novel function of adipocyte plasma membrane associated protein (APMAP) in gestational diabetes mellitus by proteomic analysis of omental adipose tissues


ABSTRACT: Gestational diabetes mellitus (GDM) is considered as the early stage of type 2 diabetes mellitus. In this study, we compared the demographic and clinical data between six GDM and six NGT (healthy controls) and found the HOMA-IR was increased in GDM. Previously, many researches had proved that omental adipose tissues dysfunction could induce insulin resistance. Thus, in order to investigate the cause of the insulin resistance in GDM, label-free proteomics was used to discover differentially expressed proteins in omental adipose tissues between GDM and NGT. A total of 3529 proteins identified, including 66 significantly changed proteins. Adipocyte plasma membrane associated protein (APMAP also called C20orf3) was one of changed proteins and down regulated in GDM omental adipose tissues. Further, mature 3T3-L1 adipocytes were used to simulate omental adipocytes and we found that inhibited the expression of APMAP by RNAi would impaired the insulin signaling and activated the NFkB signaling in adipocytes. These results indicated that the decreased of APMAP in mental adipose tissues may be important in process of the insulin resistance in GDM.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Adipose Tissue

SUBMITTER: Xing Liu  

LAB HEAD: Hu Zhou

PROVIDER: PXD003095 | Pride | 2016-02-02

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20140401_MYH_C18_15ug.raw Raw
20140401_MYH_C37_15ug.raw Raw
20140401_MYH_C64_15ug.raw Raw
20140401_MYH_C68_15ug.raw Raw
20140401_MYH_C73_15ug.raw Raw
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Publications

Identification of a Novel Function of Adipocyte Plasma Membrane-Associated Protein (APMAP) in Gestational Diabetes Mellitus by Proteomic Analysis of Omental Adipose Tissue.

Ma Yuhang Y   Gao Jing J   Yin Jiajing J   Gu Liping L   Liu Xing X   Chen Su S   Huang Qianfang Q   Lu Huifang H   Yang Yuemin Y   Zhou Hu H   Wang Yufan Y   Peng Yongde Y  

Journal of proteome research 20160127 2


Gestational diabetes mellitus (GDM) is considered as an early stage of type 2 diabetes mellitus. In this study, we compared demographic and clinical data between six GDM subjects and six normal glucose tolerance (NGT; healthy controls) subjects and found that homeostasis model of assessment for insulin resistance index (HOMA-IR) increased in GDM. Many previous studies demonstrated that omental adipose tissue dysfunction could induce insulin resistance. Thus, to investigate the cause of insulin r  ...[more]

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