Proteome-wide identification of SAPs in an industrial yeast strain

ABSTRACT: Most proteogenomic approaches for mapping single amino acid polymorphisms (SAPs) require construction of a sample-specific database containing protein variants predicted from the next-generation sequencing (NGS) data. We present a new strategy for direct SAP detection without relying on NGS data. Among the 348 putative SAP peptides identified in an industrial yeast strain, 85.6% of SAP sites were validated by genomic sequencing.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Cell Culture

SUBMITTER: Guo Yufeng  

LAB HEAD: Wenqing Shui

PROVIDER: PXD003101 | Pride | 2018-04-18

REPOSITORIES: Pride