Proteomics

Dataset Information

0

UVPD applied to phosphoproteomics

ABSTRACT: UV-photodissociation was incorporated into a phosphoproteomic workflow for the analysis of peptide PTMs.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Synthetic Construct

SUBMITTER: Kyle Fort  

LAB HEAD: Kyle L. Fort

PROVIDER: PXD003109 | Pride | 2017-08-08

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
2015-03-18_Peptide_12_100Hz_5mJ.RAW Raw
2015-03-18_Peptide_12_NCE25.RAW Raw
2015-03-21_Peptide_11_STATIC_3_3mJ.RAW Raw
2015-03-21_Peptide_11_STATIC_3_5mJ.RAW Raw
2015-03-21_Peptide_11_STATIC_3_5mJ_050_Hz.RAW Raw
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Publications

Implementation of Ultraviolet Photodissociation on a Benchtop Q Exactive Mass Spectrometer and Its Application to Phosphoproteomics.

Fort Kyle L KL   Dyachenko Andrey A   Potel Clement M CM   Corradini Eleonora E   Marino Fabio F   Barendregt Arjan A   Makarov Alexander A AA   Scheltema Richard A RA   Heck Albert J R AJ  

Analytical chemistry 20160129 4

Proteomics applications performed on the popular benchtop Q Exactive Orbitrap mass spectrometer have so far relied exclusively on higher collision-energy dissociation (HCD) fragmentation for peptide sequencing. While this fragmentation technique is applicable to a wide range of biological questions, it also has limitations, and all questions cannot be addressed equally well. Here, we demonstrate that the fragmentation capabilities of the Q Exactive mass spectrometer can be extended with ultravio  ...[more]