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Bovine milk proteome during involution

ABSTRACT: This project tested the hypothesis that drying off and involution is accompanied by similar changes among the low abundance proteins as mastitis, thereby presenting a possible alternative means of maximising yields of host-defence-associated bioactive proteins.

INSTRUMENT(S): QSTAR

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Milk

SUBMITTER: Kasper Hettinga

LAB HEAD: Thomas T. Wheeler

PROVIDER: PXD003110 | Pride | 2016-06-14

REPOSITORIES: Pride

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Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution.

Boggs Irina I Hine Brad B Smolenksi Grant G Hettinga Kasper K Zhang Lina L Wheeler Thomas T TT

Data in brief 20160513

Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic peptides followed by Orbitrap MS/MS (DML), to quantify the changes in the repertoire of bovine milk proteins that occurs after drying off. We analysed skim milk and whey sampled at day 0 and either day 3 ...[more]

PMID: 27274532

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