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Quantitative proteomics of CELF1-silenced human melanoma cell lines

ABSTRACT: CELF1 was silenced in two human melanoma cell lines (SKMEL-103 and UACC-62, indicated as 9M and 17M, respectively) using short hairpin RNA (tag 114). As control scrambled non targeting shControl transduced cell were used (tag 115).

OTHER RELATED OMICS DATASETS IN: PRJNA326505PRJNA348439PRJNA325307PRJNA326686

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Melanoma

SUBMITTER: Pilar Ximenez-Embun

LAB HEAD: Javier Muñoz Peralta

PROVIDER: PXD003112 | Pride | 2017-12-21

REPOSITORIES: Pride

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Systems analysis identifies melanoma-enriched pro-oncogenic networks controlled by the RNA binding protein CELF1.

Cifdaloz Metehan M Osterloh Lisa L Graña Osvaldo O Riveiro-Falkenbach Erica E Ximénez-Embún Pilar P Muñoz Javier J Tejedo Cristina C Calvo Tonantzin G TG Karras Panagiotis P Olmeda David D Miñana Belén B Gómez-López Gonzalo G Cañon Estela E Eyras Eduardo E Guo Haihong H Kappes Ferdinand F Ortiz-Romero Pablo L PL Rodríguez-Peralto Jose L JL Megías Diego D Valcárcel Juan J Soengas María S MS

Nature communications 20171221 1

Melanomas are well-known for their altered mRNA expression profiles. Yet, the specific contribution of mRNA binding proteins (mRBPs) to melanoma development remains unclear. Here we identify a cluster of melanoma-enriched genes under the control of CUGBP Elav-like family member 1 (CELF1). CELF1 was discovered with a distinct prognostic value in melanoma after mining the genomic landscape of the 692 known mRBPs across different cancer types. Genome-wide transcriptomic, proteomic, and RNA-immunopr ...[more]

PMID: 29269732

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