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HEK293 Quality Control example dataset - contamination test

ABSTRACT: A simple HEK293 lysate, with two files containing a putative mycoplasma contamination, and two negative control samples, taken from Geiger etc al. (Mol Cell Proteomics. 2012 Mar;11(3):M111.014050. doi: 10.1074/mcp.M111.014050. Epub 2012 Jan 25.) [PXD002395].

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Kidney

SUBMITTER: Chris Bielow

LAB HEAD: Stefan Kempa

PROVIDER: PXD003134 | Pride | 2015-11-04

REPOSITORIES: Pride

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Proteomics Quality Control: Quality Control Software for MaxQuant Results.

Bielow Chris C Mastrobuoni Guido G Kempa Stefan S

Journal of proteome research 20151228 3

Mass spectrometry-based proteomics coupled to liquid chromatography has matured into an automatized, high-throughput technology, producing data on the scale of multiple gigabytes per instrument per day. Consequently, an automated quality control (QC) and quality analysis (QA) capable of detecting measurement bias, verifying consistency, and avoiding propagation of error is paramount for instrument operators and scientists in charge of downstream analysis. We have developed an R-based QC pipeline ...[more]

PMID: 26653327

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