Dataset Information

Molecular profiling of a monkey ischemia stroke model

ABSTRACT: In this study, we examined a Macaca fascicularis transient middle cerebral artery occlusion surgery-induced stroke model, consisting of two sample groups, determined by MRI-quantified infarct volumes as a measure of the stroke severity 28 days after the ischemic episode.

INSTRUMENT(S):

ORGANISM(S): Macaca Fascicularis Fascicularis

TISSUE(S): Brain

DISEASE(S): Cerebrovascular Disease

SUBMITTER: Henry Law

LAB HEAD: Ivan K. Chu

PROVIDER: PXD003173 | Pride | 2017-01-21

REPOSITORIES: Pride

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Publications

Characterization of the Molecular Mechanisms Underlying the Chronic Phase of Stroke in a Cynomolgus Monkey Model of Induced Cerebral Ischemia.

Law Henry C H HC Szeto Samuel S W SS Quan Quan Q Zhao Yun Y Zhang Zaijun Z Krakovska Olga O Lui Leong Ting LT Zheng Chengyou C Lee Simon M-Y SM Siu K W Michael KW Wang Yuqiang Y Chu Ivan K IK

Journal of proteome research 20170202 3

Stroke is one of the main causes of mortality and long-term disability worldwide. The pathophysiological mechanisms underlying this disease are not well understood, particularly in the chronic phase after the initial ischemic episode. In this study, a Macaca fascicularis stroke model consisting of two sample groups, as determined by MRI-quantified infarct volumes as a measure of the stroke severity 28 days after the ischemic episode, was evaluated using qualitative and quantitative proteomics an ...[more]

PMID: 28102082

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Project description:Background: While stressful events are recognized as an important cause of major depressive disorder (MDD), some individuals exposed to life stressors maintain normal psychological functioning. Although the molecular mechanism(s) underlying this phenomenon remain unclear, abnormal transmission and plasticity of hippocampal synapses may play a key role in the pathoetiology of MDD. Methods: A chronic mild stress (CMS) protocol was applied to separate susceptible and unsusceptible rat subpopulations. Proteomic analysis using an isobaric tag for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry was performed to identify differential proteins in enriched hippocampal synaptic junction preparations. Results: A total of 4318 proteins were quantified, and 89 membrane proteins were present in differential amounts. Of these, SynaptomeDB identified 81 (91%) having a synapse-specific localization. The unbiased profiles identified several candidate proteins within the synaptic junction that may be associated with stress vulnerability or insusceptibility. Subsequent functional categorization revealed that protein systems particularly involved in membrane trafficking at the synaptic active zone exhibited a positive strain as potential molecular adaptations in the unsusceptible rats. Moreover, through STRING and immumoblotting analysis, membrane-associated GTP-bound Rab3a and Munc18-1 appear to co-regulate syntaxin-1/SNAP25/VAMP2 assembly at the hippocampal presynaptic active zone of unsusceptible rats, facilitating SNARE-mediated membrane fusion and neurotransmitter release, and may be part of a stress-protection mechanism in actively maintaining an emotional homeostasis. Conclusions: The present results support the concept that there are a range of potential protein adaptations in the hippocampal synaptic active zone of unsusceptible rats, revealing new investigative targets that may contribute to a better understanding of stress insusceptibility.

			Action	DRS
	I01.group	Other
	I01_3h_A1_20121208.rar	Other
	I01_MGFPeaklist.mgf	Mgf
	I01_PeptideSummary.txt	Txt
	I01_ProteinSummary.txt	Txt