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Identification of FLS2 interacting proteins by co-immunoprecipitation and shot gun proteomics

ABSTRACT: To identify immediate early signaling components in the FLS2 pathway we performed co-immunoprecipitation (co-IP) experiments with FLS2-GFP as bait in Arabidopsis. We immunoprecipitated FLS2-GFP with anti-GFP antibody coated beads and analyzed co-precipitated proteins by liquid chromatography tandem mass spectrometry (LC-MS/MS).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Seedling

SUBMITTER: Jan Sklenar

LAB HEAD: Frank Menke

PROVIDER: PXD003189 | Pride | 2016-01-26

REPOSITORIES: Pride

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Attenuation of pattern recognition receptor signaling is mediated by a MAP kinase kinase kinase.

Mithoe Sharon C SC Ludwig Christina C Pel Michiel J C MJ Cucinotta Mara M Casartelli Alberto A Mbengue Malick M Sklenar Jan J Derbyshire Paul P Robatzek Silke S Pieterse Corné M J CM Aebersold Ruedi R Menke Frank L H FL

EMBO reports 20160114 3

Pattern recognition receptors (PRRs) play a key role in plant and animal innate immunity. PRR binding of their cognate ligand triggers a signaling network and activates an immune response. Activation of PRR signaling must be controlled prior to ligand binding to prevent spurious signaling and immune activation. Flagellin perception in Arabidopsis through FLAGELLIN-SENSITIVE 2 (FLS2) induces the activation of mitogen-activated protein kinases (MAPKs) and immunity. However, the precise molecular m ...[more]

PMID: 26769563

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