Dataset Information

Rickettsia massiliae intra cellular bacteria global proteome

ABSTRACT: Semi quantitative intracellular bacteria proteome using a 2D nanoLC-M/MS analysis system enabling a maximum sample exploration. This setup was helpful to analyse this complex sample composed of peptides from the studied bacteria and its host. Sample was doped before injection in order to calculate protein quantities using the Hi3 absolute quantification technique.

INSTRUMENT(S): Synapt G2-S HDMS

ORGANISM(S): Rickettsia Massiliae (strain Mtu5) Xenopus Laevis (african Clawed Frog)

SUBMITTER: ARMSTRONG Nicholas

LAB HEAD: Eric Chabriere

PROVIDER: PXD003197 | Pride | 2018-10-25

REPOSITORIES: Pride

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Publications

Multi-omics Analysis Sheds Light on the Evolution and the Intracellular Lifestyle Strategies of Spotted Fever Group Rickettsia spp.

El Karkouri Khalid K Kowalczewska Malgorzata M Armstrong Nicholas N Azza Said S Fournier Pierre-Edouard PE Raoult Didier D

Frontiers in microbiology 20170720

Arthropod-borne Rickettsia species are obligate intracellular bacteria which are pathogenic for humans. Within this genus, Rickettsia slovaca and Rickettsia conorii cause frequent and potentially severe infections, whereas Rickettsia raoultii and Rickettsia massiliae cause rare and milder infections. All four species belong to spotted fever group (SFG) rickettsiae. However, R. slovaca and R. raoultii cause scalp eschar and neck lymphadenopathy (SE ...[more]

PMID: 28775717

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Project description:Background: Canmei formula (CMF) is a traditional Chinese medicine compound with definite effect on the prevention and treatment of colorectal adenoma (CRA). It can prevent the transformation of intestinal inflammation to cancer. This study explored the mechanism of action of CMF in anti-CRA using multi-omics techniques. Method: Mice were randomly divided into 4 groups: blank group (Control), high-fat diet (HFD) + AOM/DSS colorectal adenoma model (ADH) groups, Canmei formula treatment group (ADH-CMF) and sulfasalazine treatment group (Sul). Except for the blank group, ADH model was established in the other 3 groups by intraperitoneal injection with AOM reagent, and then mice was given 2.5%DSS in free drinking water and high-fat diet. The mice in the blank group and ADH group were intragastrically perfused with normal saline, and the mice in the other 2 groups were treated with corresponding drugs for 20 weeks. During this period, the changes of physical signs of mice in each group were observed. The differentially expressed genes and proteins in the Control group, ADH group and ADH-CMF group were detected by RNA-seq transcriptome sequencing and Tandem Mass Tags (TMT) quantitative proteomics. After the combined analysis and verification. The key targets were analyzed by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Results : A total of 2548 differential genes were obtained by transcriptomics analysis, and 45 differential proteins were obtained by proteomics analysis. The results of proteomics data and experimental verification showed that CMF mainly acted on the Phospholysine Phosphohistidine Inorganic Pyrophosphate Phosphatase (LHPP) target. GO analysis showed that the targets of CMF were involved in the biological processes such as cellular process, metabolic process and biological regulation. KEGG analysis showed that those genes were involved in oxidative phosphorylation, cell senescence, and Metabolic pathways. Studies have shown that Lhpp overexpression of Lhpp impeded Colorectal cancer cell growth and proliferation in vitro, and was associated with a change in PI3K/AKT activity.

Dataset Information

Rickettsia massiliae intra cellular bacteria global proteome

Publications

Multi-omics Analysis Sheds Light on the Evolution and the Intracellular Lifestyle Strategies of Spotted Fever Group <i>Rickettsia</i> spp.

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