Known ratios of SILAC labels in E. Coli
Ontology highlight
ABSTRACT:
INSTRUMENT(S):
ORGANISM(S): Escherichia Coli
SUBMITTER: 
Akhilesh Pandey
LAB HEAD: Akhilesh Pandey
PROVIDER: PXD003327 | Pride | 2016-03-01
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Chris_Ecoli_1-1-01.msf | Msf | |||
| Chris_Ecoli_1-1.mzML | Mzml | |||
| Chris_Ecoli_1-2-4-01.msf | Msf | |||
| Chris_Ecoli_1-2-4.mzML | Mzml | |||
| Chris_Ecoli_4-1-01.msf | Msf |
Items per page: 5 1 - 5 of 6 |
Similar Datasets
Project description:3 raw datasets corresponding to known mixtures of lysine and arginine in E. Coli.
2016-08-16 | PXD003845 | Pride
Project description:Forum domains are stretches of chromosomal DNA that are excised from eukaryotic chromosomes during their spontaneous non-random fragmentation. Mostly forum domains are of 50-200 kb in length, although larger domains, up to 500 - 700 kb, are also observed. <br><br>We performed a genome-wide mapping of forum domains termini in Drosophila Schneider 2 cultured cells using Drosophila melanogaster genomic tiling microarray consisting of overlapping 3 kb fragments. We found that forum domains contain clusters of several or many genes inside. The largest forum domains correspond to the coordinately expressed main clusters of homeotic genes inside BX-C and ANTP-C, cluster of histone genes and clusters of piRNAs. PRE/TRE and binding sites of transcription factors are often reside inside domains, and do not overlap with forum domains termini. The terminal regions of domains often correspond to chromosomal sites containing different classes of mobile elements, binding sites of SuUR protein and regions of intercalary heterochromatin. Our results indicate that forum domains correspond to big multi-gene chromosomal units some of which could be co-coordinately activated or repressed.
2010-11-01 | E-MEXP-2468 | biostudies-arrayexpress
Project description:MS treated wild-type Arabidopsis seedlings versus MS Rose Bengal (RB)-containing medium (10 microM) wild type treated seedlings
2011-03-01 | E-MEXP-3009 | biostudies-arrayexpress
Project description:Global kinase activity induced by cytokines IL-32g and IL-17A/F were determined using peptide arrays representing phophorylation targets The objective of this study was to identify common and unique phosphorylation targets of pro-inflammatory cytokines IL-32 and IL-17. These cytokines are associated with the pathogenesis and severity of chronic inflammatory disorders, therefore signaling intermediates of these cytokines could be beneficial as alternate theraputic targets that may likely influence different inflammatory pathways. Phosphorylation of proteins is a critical mechanism in the regulation of cellular processes. This process is meticulously regulated by enzymes known as kinases, which are increasingly being identified as drug targets for a variety of diseases. In this study we interrogated kinase activities (kinome) induced in the presence of the human recombinant cytokines IL-32g and IL-17A/F employing peptide arrays representing 300 peptides, printed in triplicate, representing select phosphorylation events. Human macrophage-like THP-1 cells were used for this comparative kinome analysis. Macrophage-like THP-1 cells were stimulated with either IL-32g (20 ng/ml) or IL-17A/F (20 ng/ml) for 15 min, and the peptide arrays were used to comprehensively analyze protein phosphorylation profiles in the presence of these cytokines. Two independet biological experiments were performed and the kinoem analysis was done in triplicates for each. The phosphorylations of the peptides on the array were quantified in the cytokine-treated cells relative to the un-stimulated control cells. Differentially phosphorylated targets were defined as greater than 1.5 fold increase or decrease (p < 0.06) in phosphorylation compared to un-stimulated control cells.
2011-04-16 | E-GEOD-28649 | biostudies-arrayexpress
Project description:Background: It has been shown previously that administration of Francisella tularensis (Ft) LVS lipopolysaccharide (LPS) protects mice against subsequent challenge with Ft LVS and blunts the pro-inflammatory cytokine response. Methods: To investigate further the molecular mechanisms that underlie Ft LVS LPS-mediated protection, we profiled global hepatic gene expression following Ft LVS LPS or saline pretreatment and subsequent Ft LVS challenge using Affymetrix arrays. Results: A large number of genes (> 3000) were differentially expressed at 48 hours post-infection. The degree of modulation of inflammatory genes by infection was clearly attenuated by LPS-pretreatment in the surviving mice. However, LPS alone had a subtle but significant effect on the gene expression profile of the uninfected mice. By employing gene set enrichment analysis, we discovered significant up-regulation of peroxisome proliferator activated receptors (PPARs) and their target genes in LPS-treated liver. Conclusions: We hypothesize that the LPS-induced blunting of pro-inflammatory response in mouse is, in part, mediated by PPARs (alpha and gamma). Experiment Overall Design: Two groups of 9 mice each were used for this experiment. 48 hours prior to Ft LVS challenge, mice were injected i.p. with either 100 ng of Ft LVS LPS or an equivalent volume of saline. On the day of challenge, 3 saline- and 3 Ft LVS LPS-pretreated animals were sacrificed (uninfected controls), while all remaining mice were challenged i.p. with ~4-5 X 105 Ft LVS. Ft LVS-challenged mice were sacrificed (in groups of 3) at 24 and 48 hours post-infection.
2010-02-21 | E-GEOD-16207 | biostudies-arrayexpress
Project description:Skeletal growth promoted by endochondral ossification is tightly coordinated by self-renewal and differentiation of chondrogenic progenitors. Emerging evidence has shown that multiple skeletal stem cells (SSCs) participate in cartilage formation. However, as yet, no study has reported the existence of common long-lasting chondrogenic progenitors in various types of cartilage. Here, we identified Gli1+ chondrogenic progenitors (Gli1+ CPs), which were distinct from SSCs, were responsible for the lifelong generation of chondrocytes in the growth plate, vertebrae, ribs, and other cartilage. The absence of Gli1+ CPs led to cartilage defects and dwarfishness phenotype in mice. Furthermore, we found that the BMP signal played an important role in self-renewal and maintenance of Gli1+ CPs. The deletion of Bmpr1α caused the exhaustion of Gli1+ CPs, consequently disrupting columnar cartilage. Collectively, our data demonstrate that Gli1+ CPs are common long-term chondrogenic progenitors in multiple types of cartilage and are essential to maintain cartilage homeostasis.
2024-02-18 | GSE249831 | GEO
Project description:Rose Bengal-treated wild-type seedlings versus Rose Bengal-treated eto1-14 mutant
2011-03-01 | E-MEXP-3011 | biostudies-arrayexpress
Project description:Rose Bengal-treated wild type seedlings versus Rose Bengal-treated lox1lox5 mutant
2011-03-01 | E-MEXP-3010 | biostudies-arrayexpress
Project description:PUF2 RNAi, V5-PUF2 and UBP1-myc pull-downs, all methods and protocols are described in detail in the publication
2018-05-21 | E-MTAB-5617 | biostudies-arrayexpress
Project description:Transcriptome changes in leaves of PcG protein mutants clf and lhp1. Fully expanded rosette leaf 6 was collected from 10 35-d-old plants grown in short day photoperiods.
2013-08-22 | E-MTAB-1412 | biostudies-arrayexpress