Tandem affinity purification and Mass spectrometry (TAP-MS) analysis of PCH1’s associated proteins in various Arabidopsis genetic backgrounds.

ABSTRACT: Here we describe a series TAP-MS analyses in various mutant backgrounds of Arabidopsis thaliana to co-purify any PCH1’s associated proteins, using tagged PCH1 as bait (PCH1-His6-Flag3, either overexpressed or under the control of its own promoter). By combining TAP-MS with genetics, we investigate the in-vivo dependence of several proteins for maintaining the association of PCH1 with its interacting proteins. Our results refine the protein-protein interaction network of an existing interactome containing phytochromes, the COP1-SPAs complex and the ELF3-ELF4-LUX complex.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Sophe Alvarez  

LAB HEAD: Meter Nusinow

PROVIDER: PXD003352 | Pride | 2016-02-09

REPOSITORIES: Pride