Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Escherichia Coli
SUBMITTER: Chao Ji
LAB HEAD: James P. Reilly
PROVIDER: PXD003381 | Pride | 2015-12-23
REPOSITORIES: Pride
Journal of proteome research 20110624 8
The structure of the Escherichia coli ribosome, a 2.5 MDa ribonucleoprotein complex containing more than 50 proteins, was probed using the novel amidinating cross-linker diethyl suberthioimidate (DEST) and mass spectrometry. Peptide cross-links derived from this complex structure were identified at high confidence (FDR 0.8%) from precursor mass measurements and collision-induced dissociation (CID) fragmentation spectra. The acquired cross-linking data were found to be in excellent agreement with ...[more]