CEP215_gallus_interactome - A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer

ABSTRACT: To establish the molecular basis for CEP215 function in centrosome-spindle pole attachment, we employed an unbiased proteomic approach to isolat e and identify CEP215 interactors. To this end, affinity purification tags ( Gs TAP containing protein G and streptavidin-binding protein) were inserted in-frame into both alleles of the CEP215 gene (CEP215-TAP cell line) in the chicken B cell line, DT40. Following affinity purification, protein complexes were analysed by mass spectr ometry

INSTRUMENT(S):

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): B Cell

SUBMITTER: Pavithra Lakshminarasimhan  

LAB HEAD: Fanni gergely

PROVIDER: PXD003382 | Pride | 2016-03-31

REPOSITORIES: Pride