Proteomics

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CEP215_gallus_interactome - A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer

ABSTRACT: To establish the molecular basis for CEP215 function in centrosome-spindle pole attachment, we employed an unbiased proteomic approach to isolat e and identify CEP215 interactors. To this end, affinity purification tags ( Gs TAP containing protein G and streptavidin-binding protein) were inserted in-frame into both alleles of the CEP215 gene (CEP215-TAP cell line) in the chicken B cell line, DT40. Following affinity purification, protein complexes were analysed by mass spectr ometry

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): B Cell

SUBMITTER: Pavithra Lakshminarasimhan  

LAB HEAD: Fanni gergely

PROVIDER: PXD003382 | Pride | 2016-03-31

REPOSITORIES: Pride

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A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer.

Chavali Pavithra L PL   Chandrasekaran Gayathri G   Barr Alexis R AR   Tátrai Péter P   Taylor Chris C   Papachristou Evaggelia K EK   Woods C Geoffrey CG   Chavali Sreenivas S   Gergely Fanni F  

Nature communications 20160318

Numerical centrosome aberrations underlie certain developmental abnormalities and may promote cancer. A cell maintains normal centrosome numbers by coupling centrosome duplication with segregation, which is achieved through sustained association of each centrosome with a mitotic spindle pole. Although the microcephaly- and primordial dwarfism-linked centrosomal protein CEP215 has been implicated in this process, the molecular mechanism responsible remains unclear. Here, using proteomic profiling  ...[more]

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