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Bovine sperm surface lectinome

ABSTRACT: Identification of bovine sperm surface carbohydrate-binding proteins relevant in two important aspects of fertilization: (i) formation of the sperm reservoir in the oviductal epithelium, and (ii) gamete recognition (oocyte-sperm interaction). Using whole sperm cells and a novel affinity capture method that combines proteolysis of protein-glycan complexes and mass spectrometry (i.e., CREDEX-MS), several lectins were enriched, identified by MS/MS proteomics, and mapped within the fertilization events in bovine species.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Semen, Sperm

SUBMITTER: Sira Defaus

LAB HEAD: David Andreu

PROVIDER: PXD003386 | Pride | 2016-04-28

REPOSITORIES: Pride

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Identification of Bovine Sperm Surface Proteins Involved in Carbohydrate-mediated Fertilization Interactions.

Defaus Sira S Avilés Manuel M Andreu David D Gutiérrez-Gallego Ricardo R

Molecular & cellular proteomics : MCP 20160419 7

Glycan-protein interactions play a key role in mammalian fertilization, but data on the composition and identities of protein complexes involved in fertilization events are scarce, with the added complication that the glycans in such interactions tend to differ among species. In this study we have used a bovine model to detect, characterize and identify sperm lectins relevant in fertilization. Given the complexity of the sperm-toward-egg journey, two important aspects of the process, both primar ...[more]

PMID: 27094474

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