Proteomic analysis of glutamine deprivation in HepG2 cells

ABSTRACT: We applied the quantitative proteomics approach to estimate the relative abundance of proteins in HepG2 cells treated by glutamine deprivation. Two independent cultured cell lysates, namely control and glutamine deprivation, were marked with iTRAQ labels and subjected to LC-ESI-MS/MS analysis. The tryptic peptides of two samples were labeled with mass 113 (control) and 119 (glutamine deprivation) isobaric iTRAQ tags.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Hepg2 Cell

DISEASE(S): Hepatocellular Carcinoma

SUBMITTER: Baisheng Long  

LAB HEAD: Xianghua Yan

PROVIDER: PXD003387 | Pride | 2016-02-09

REPOSITORIES: Pride