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Mitotic chromatin proteomics - Proteomics analysis with a nano Random Forest approach reveals novel functional interactions regulated by SMC complexes on mitotic chromosomes

ABSTRACT: we used stable isotope labeling with amino acids in cell culture (SILAC) to compare the proteomes of mitotic chromosomes isolated from cell lines harboring conditional knockouts of members of the condensin (SMC2, CAP-H, CAP-D3), cohesin (Scc1/Rad21), and SMC5/6 (SMC5) complexes.

INSTRUMENT(S): LTQ Orbitrap, LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): B Cell

SUBMITTER: Shinya Ohta

LAB HEAD: SHINYA OHTA

PROVIDER: PXD003427 | Pride | 2016-05-31

REPOSITORIES: Pride

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Proteomics Analysis with a Nano Random Forest Approach Reveals Novel Functional Interactions Regulated by SMC Complexes on Mitotic Chromosomes.

Ohta Shinya S Montaño-Gutierrez Luis F LF de Lima Alves Flavia F Ogawa Hiromi H Toramoto Iyo I Sato Nobuko N Morrison Ciaran G CG Takeda Shunichi S Hudson Damien F DF Rappsilber Juri J Earnshaw William C WC

Molecular & cellular proteomics : MCP 20160526 8

Packaging of DNA into condensed chromosomes during mitosis is essential for the faithful segregation of the genome into daughter nuclei. Although the structure and composition of mitotic chromosomes have been studied for over 30 years, these aspects are yet to be fully elucidated. Here, we used stable isotope labeling with amino acids in cell culture to compare the proteomes of mitotic chromosomes isolated from cell lines harboring conditional knockouts of members of the condensin (SMC2, CAP-H, ...[more]

PMID: 27231315

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