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Proteome profiling of Bacillus subtilis stressed with the antibiotics tetracycline and chelocardin

ABSTRACT: Chelocardin is an atypical tetracycline and its mechanism of action is discussed controversially in literature. Therefore, we analyzed the bacterial response of Bacillus subtilis to chelocardin and as a control for classical tetracyclines to tetracycline by proteomic profiling. The induced marker proteins mirror the damage in the cell due the antibiotic stress. Proteomic profiling was carried out by 2D-PAGE and spot identification by nUPLC-ESI-MS/MS. The proteomic profile of chelocardin demonstrates a dual mechanism of action with protein biosynthesis inhibition and membrane damage.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168

SUBMITTER: Jennifer Stepanek

LAB HEAD: Julia Elisabeth Bandow

PROVIDER: PXD003439 | Pride | 2016-03-14

REPOSITORIES: Pride

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Dual mechanism of action of the atypical tetracycline chelocardin.

Stepanek Jennifer J JJ Lukežič Tadeja T Teichert Ines I Petković Hrvoje H Bandow Julia E JE

Biochimica et biophysica acta 20160309 6

Classical tetracyclines targeting the protein biosynthesis machinery are commonly applied in human and veterinary medicine. The development and spread of resistance seriously compromise the successful treatment of bacterial infections. The atypical tetracycline chelocardin holds promise as it retains activity against tetracycline-resistant strains. It has been suggested that chelocardin targets the bacterial membrane, thus differing in mode of action from that of classical tetracyclines. We inve ...[more]

PMID: 26969785

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