Proteome profiling of Bacillus subtilis stressed with the antibiotics tetracycline and chelocardin

ABSTRACT: Chelocardin is an atypical tetracycline and its mechanism of action is discussed controversially in literature. Therefore, we analyzed the bacterial response of Bacillus subtilis to chelocardin and as a control for classical tetracyclines to tetracycline by proteomic profiling. The induced marker proteins mirror the damage in the cell due the antibiotic stress. Proteomic profiling was carried out by 2D-PAGE and spot identification by nUPLC-ESI-MS/MS. The proteomic profile of chelocardin demonstrates a dual mechanism of action with protein biosynthesis inhibition and membrane damage.

INSTRUMENT(S):

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168

SUBMITTER: Jennifer Stepanek  

LAB HEAD: Julia Elisabeth Bandow

PROVIDER: PXD003439 | Pride | 2016-03-14

REPOSITORIES: Pride