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The interactome of the human RNA Pol II

ABSTRACT: We aim to describe the proteins interacting with the human RNA polymerase II

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell

SUBMITTER: Ignasi Forne

LAB HEAD: Axel Imhof

PROVIDER: PXD003459 | Pride | 2018-01-09

REPOSITORIES: Pride

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Specific threonine-4 phosphorylation and function of RNA polymerase II CTD during M phase progression.

Hintermair Corinna C Voß Kirsten K Forné Ignasi I Heidemann Martin M Flatley Andrew A Kremmer Elisabeth E Imhof Axel A Eick Dirk D

Scientific reports 20160606

Dynamic phosphorylation of Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7 heptad-repeats in the C-terminal domain (CTD) of the large subunit coordinates progression of RNA polymerase (Pol) II through the transcription cycle. Here, we describe an M phase-specific form of Pol II phosphorylated at Thr4, but not at Tyr1, Ser2, Ser5, and Ser7 residues. Thr4 phosphorylated Pol II binds to centrosomes and midbody and interacts with the Thr4-specific Polo-like kinase 1. Binding of Pol II to centrosomes does not req ...[more]

PMID: 27264542

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