DIFFERENTIAL NEUROPROTEOMIC AND SYSTEMS BIOLOGY ANALYSIS OF SPINAL CORD INJURY
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ
ORGANISM(S): Rattus Rattus (black Rat)
TISSUE(S): Brain, B Cell
DISEASE(S): Neuroblastoma
SUBMITTER: 
Ahmed Moghieb
LAB HEAD: Kevin Wang
PROVIDER: PXD003473 | Pride | 2016-05-10
REPOSITORIES: Pride
Publications
Differential Neuroproteomic and Systems Biology Analysis of Spinal Cord Injury.
Moghieb Ahmed A Bramlett Helen M HM Das Jyotirmoy H JH Yang Zhihui Z Selig Tyler T Yost Richard A RA Wang Michael S MS Dietrich W Dalton WD Wang Kevin K W KK
Molecular & cellular proteomics : MCP 20160505 7
Acute spinal cord injury (SCI) is a devastating condition with many consequences and no known effective treatment. Although it is quite easy to diagnose traumatic SCI, the assessment of injury severity and projection of disease progression or recovery are often challenging, as no consensus biomarkers have been clearly identified. Here rats were subjected to experimental moderate or severe thoracic SCI. At 24h and 7d postinjury, spinal cord segment caudal to injury center versus sham samples was ...[more]
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Project description:Spinal cord injury (SCI) represents a major debilitating health issue with a direct socioeconomic burden on the public and private sectors worldwide. Although several studies have been conducted to identify the molecular progression of injury sequel due from the lesion site, still the exact underlying mechanisms and pathways of injury development have not been fully elucidated. In this work, based on OMICs, 3D MALDI imaging, cytokines arrays, confocal imaging we established for the first time that molecular and cellular processes occurring after spinal cord injury (SCI) are altered between the lesion proximity, i.e., rostral and caudal segments nearby the lesion (R1-C1) whereas segments distant from R1-C1, i.e., R2-C2 and R3-C3 levels co-expressed factors implicated in neurogenesis. Delay in T regulators recruitment between R1 and C1 favor discrepancies between the two segments. This is also reinforced by presence of neurites outgrowth inhibitors in C1, absent in R1. Moreover, the presence of immunoglobulins (IgGs) in neurons at the lesion site at 3 days, validated by mass spectrometry, may present additional factor that contributes to limited regeneration. Treatment in vivo with anti-CD20 one hour after SCI did not improve locomotor function and IgG expression. These results open the door of a novel view of the SCI treatment by considering the C1 as the therapeutic target.
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