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Rat spleen proteomics profiling

ABSTRACT: Sample preparation optimisation for extracting in a quantitative manner proteins from rat spleen

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Spleen

SUBMITTER: Thierry Le Bihan

LAB HEAD: Thierry Le Bihan

PROVIDER: PXD003520 | Pride | 2016-07-04

REPOSITORIES: Pride

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Data from quantitative label free proteomics analysis of rat spleen.

Dudekula Khadar K Le Bihan Thierry T

Data in brief 20160604

The dataset presented in this work has been obtained using a label-free quantitative proteomic analysis of rat spleen. A robust method for extraction of proteins from rat spleen tissue and LC-MS-MS analysis was developed using a urea and SDS-based buffer. Different fractionation methods were compared. A total of 3484 different proteins were identified from the pool of all experiments run in this study (a total of 2460 proteins with at least two peptides). A total of 1822 proteins were identified ...[more]

PMID: 27358910

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