Name: Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells
Keywords: Adam12

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Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells

ABSTRACT: It has been found that A Disintergrin And Metalloproteinase 12 (ADAM12) is significantly higher in multiple tumors and it has been used as a prognostic marker for cancer progression. Several ADAM12 substrates have been identified biochemically and the role of -its proteolytic function has been explored. However, its non-proteolytic function and its interacting partners have not been systematically studied. In this work, we used a MS-based quantitative proteomic approach to identify the interacting partners of the full length ADAM12 in a cervical cancer cell line. ThroughAfter bioinformatic analyses and biochemical experiments, we found that ADAM12 could regulate its interacting partners through its non-enzymatic function, such as leading to the enhanced expression of vimentin, a mesenchymal marker. In addition, the stability of ADAM12 itself was enhanced by myoferlin, which regulates cancer cell proliferation and invasion. Together, with previous findings, ADAM12 may be an intermediate molecule that links the high expression of myoferlin and vimentin in cancer progression. This result may reveal the potential non-proteolytic function of ADAM12 in cancer cellularcell biology. The information obtained here may be used to develop new strategies to alter the signaling pathways involving the non-catalytic function of ADAM12.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Early Embryonic Cell, Hela Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: yanqing zhou

LAB HEAD: Guoqiang Xu

PROVIDER: PXD003560 | Pride | 2016-07-21

REPOSITORIES: Pride

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Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells.

Zhou Yanqing Y Xiong Lipeng L Zhang Yang Y Yu Rong R Jiang Xiaogang X Xu Guoqiang G

Journal of proteomics 20160716

<h4>Unlabelled</h4>A Disintegrin and Metalloproteinase 12 (ADAM12) is expressed significantly higher in multiple tumors than in normal tissues and has been used as a prognostic marker for the evaluation of cancer progression. Although several ADAM12 substrates have been identified biochemically and its proteolytic function has been explored, the upstream regulators and the interacting proteins have not been systematically investigated. Here, we use immunoprecipitation and mass spectrometry (MS)- ...[more]

PMID: 27432471

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Dataset Information

Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells

Publications

Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells.

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