Proteomics

Dataset Information

0

Identification of Glycoproteins Associated with HIV Latently Infected Cells Using Quantitative Glycoproteomics


ABSTRACT: HIV infection is not curable due to viral latency. Compelling reports studying proteins such as CD2, PD-1, LAG-3 and TIGIT suggest that there is a distinct profile of surface proteins that can be used for targeting latently infected cells. We have recently reported that glycoproteins were differentially secreted from HIV latently infected ACH-2 cells compared to the parental A3.01 cells. This observation suggests that glyco-phenotype might be different in these two cell lines. To determine the difference, the ACH-2 and A3.01 cell lines were subjected to a glycoproteomic analysis. A total number of 940 unique N-linked glycosite-containing peptides from 515 glycoproteins were identified. Among the glycoproteins, 365 and 104 were annotated as cell surface and membrane-associated proteins, respectively. Label free quantitative LC-MS/MS analysis revealed a change of 236 glycosite-containing peptides from 172 glycoproteins between the two cell lines without reactivation. Bioinformatic analysis suggests that cell adhesion, immune response, glycoprotein metabolic process, cell motion and cell activation were associated with the changed proteins. After reactivation of latency by phorbol myristate acetate (PMA), changes in glycosite-containing peptides were observed in both cell lines. Changes in 49 glycosite-containing peptides from 45 glycoproteins might be due to viral replication. The changed proteins suggest that cell migration, response to wounding and immune response were impaired in reactivated latently infected cells. Our study provides important glycoproteomic data in respect to HIV latently infected cells. Glycoproteomics merits future application using primary cells to discover reveal mechanisms in HIV pathogenesis.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): T Cell, Cell Culture

DISEASE(S): Human Immunodeficiency Virus Infectious Disease

SUBMITTER: Weiming Yang  

LAB HEAD: Hui Zhang

PROVIDER: PXD003561 | Pride | 2016-05-23

REPOSITORIES: Pride

altmetric image

Publications

Identification of glycoproteins associated with HIV latently infected cells using quantitative glycoproteomics.

Yang Weiming W   Jackson Brooks B   Zhang Hui H  

Proteomics 20160608 13


HIV infection is not curable due to viral latency. Compelling reports suggest that there is a distinct profile of surface proteins that can be used for targeting latently infected cells. We have recently reported that glycoproteins were differentially secreted from HIV latently infected ACH-2 cells compared to the parental A3.01 cells. This finding suggests that glyco-phenotype might be different in these two cell lines. To determine the difference, the ACH-2 and A3.01 cell lines were subjected  ...[more]

Similar Datasets

2017-03-31 | PXD005080 | Pride
2022-02-23 | PXD005232 | Pride
2019-11-08 | PXD005143 | Pride
2023-10-24 | PXD024014 | Pride
2022-02-28 | PXD004495 | Pride
2023-12-28 | GSE242997 | GEO
| E-GEOD-40550 | biostudies-arrayexpress
2023-09-05 | GSE235229 | GEO
2021-08-12 | GSE156072 | GEO
2021-11-22 | GSE189314 | GEO