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Ovarian Cancer Proteomics of FFPE Tissue

ABSTRACT: We have performed in-depth examination of single tissue sections from a collection of FFPE and Frozen treated tumors, as well as cell line material all originating from different subtypes of epithelial ovarian cancer (high-grade serous, clear cell, endometrioid). Data were prepared using a tandem mass tag 10plex protocol with MS3 analysis on an Orbitrap Fusion.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Ovary, Ovary Cancer Cell

DISEASE(S): Malignant Neoplasm Of Ovary

SUBMITTER: Christopher Hughes

LAB HEAD: Gregg Morin

PROVIDER: PXD003607 | Pride | 2016-10-13

REPOSITORIES: Pride

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			Action	DRS
	SupplementalScripts_DataObjects_Methods.zip	Other
	ch_04Aug2015_OvCcell-TMT6_HpH_repA1_1.raw	Raw
	ch_04Aug2015_OvCcell-TMT6_HpH_repA1_10.raw	Raw
	ch_04Aug2015_OvCcell-TMT6_HpH_repA1_2.raw	Raw
	ch_04Aug2015_OvCcell-TMT6_HpH_repA1_3.raw	Raw

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Publications

Quantitative Profiling of Single Formalin Fixed Tumour Sections: proteomics for translational research.

Hughes Christopher S CS McConechy Melissa K MK Cochrane Dawn R DR Nazeran Tayyebeh T Karnezis Anthony N AN Huntsman David G DG Morin Gregg B GB

Scientific reports 20161007

Although re-sequencing of gene panels and mRNA expression profiling are now firmly established in clinical laboratories, in-depth proteome analysis has remained a niche technology, better suited for studying model systems rather than challenging materials such as clinical trial samples. To address this limitation, we have developed a novel and optimized platform called SP3-Clinical Tissue Proteomics (SP3-CTP) for in-depth proteome profiling of practical quantities of tumour tissues, including fo ...[more]

PMID: 27713570

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