ON THE ACCURACY, PRECISION AND STATISTICAL SIGNIFICANCE OF COMPRESSED RATIOS IN ISOBARIC LABELLING

ABSTRACT: Despite the outstanding advantages that isobaric labelling has to offer in quantitative proteomics, its precision and accuracy are undermined by ratio compression, which is consequence of the co-isolation of peptides within a selected isolation window. Here, using the two-proteomes approach we investigated the relationships between precision and accuracy on the statistical significance of peptide and protein ratios in three types of mass spectrometers.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

SUBMITTER: Ana Martinez  

LAB HEAD: Javier Muñoz Peralta

PROVIDER: PXD003640 | Pride | 2016-08-08

REPOSITORIES: Pride