Proteomics

Dataset Information

63

Probing the protein inventory of the human pathogen Clostridium difficile


ABSTRACT: The anaerobic and spore-forming bacterium Clostridium difficile has turned into an intensively studied species which can be attributed to increasing numbers of infections and rising costs for the health care system. This dataset represents a benchmark proteome of reference strain C. difficile 630erm and provides mass spectrometry based details on identified proteins which was generally missing from hitherto published datasets. An elaborate annotation and visualization of the 3764 open reading frames will serve as a valuable base for researchers trying to evaluate results of global expression studies. Furthermore, protein expression of late exponentially growing cells in the complex medium BHI and in C. difficile minimal medium was compared. Whereas abundance of proteins of DNA metabolism, protein synthesis and cell envelope showed no variation, enzymes for the biosynthesis of some vitamins and purine as well as proteins involved in butanoate fermentation differed significantly depending on the growth medium.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Clostridioides difficile 630  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Not Available

SUBMITTER: Andreas Otto  

LAB HEAD: Andreas Otto

PROVIDER: PXD003659 | Pride | 2016-08-12

REPOSITORIES: pride

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