Identification of type strain-specific diagnostic peptides of the Bacillus cereus group

ABSTRACT: The Bacillus cereus group consists of eight very closely related species and comprises both harmless and human pathogenic species. Yet, methods to rapidly and accurately distinguish these species are currently lacking as we demonstrate that classical matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) biotyping did not achieve reliable identification of each type strain. We assigned type strain-specific diagnostic peptides to the B. cereus group based on comparisons of their proteome profiles. The number of diagnostic peptides varies remarkably in type strain-dependent manner. The state of the art of the reference database is crucial in the process of validating candidate diagnostic peptides and may lead to a noteworthy reduction of verified diagnostic peptides as putative diagnostic peptides might be found in other species as well.

INSTRUMENT(S):

ORGANISM(S): Bacillus Pseudomycoides Dsm 12442 Bacillus Thuringiensis Serovar Berliner Atcc 10792 Bacillus Weihenstephanensis Nbrc 101238 = Dsm 11821 Bacillus Toyonensis Bct-7112 Bacillus Mycoides Dsm 2048 Bacillus Cytotoxicus (strain Dsm 22905 / Cip 110041 / 391-98 / Nvh 391-98) Bacillus Cereus (strain Atcc 14579 / Dsm 31)

TISSUE(S): Cell Suspension Culture

SUBMITTER: David Drissner  

LAB HEAD: David Drissner

PROVIDER: PXD003669 | Pride | 2018-02-07

REPOSITORIES: Pride