Ontology highlight
ABSTRACT:
INSTRUMENT(S): Bruker Daltonics solarix series
ORGANISM(S): Bos Taurus (bovine)
SUBMITTER: Timo Sachsenberg
LAB HEAD: Oliver Kohlbacher
PROVIDER: PXD003673 | Pride | 2017-09-11
REPOSITORIES: Pride
Flett Fiona J FJ Sachsenberg Timo T Kohlbacher Oliver O Mackay C Logan CL Interthal Heidrun H
Analytical chemistry 20171013 21
Cross-linking of nucleic acids to proteins in combination with mass spectrometry permits the precise identification of interacting residues between nucleic acid-protein complexes. However, the mass spectrometric identification and characterization of cross-linked nucleic acid-protein heteroconjugates within a complex sample is challenging. Here we establish a novel enzymatic differential <sup>16</sup>O/<sup>18</sup>O-labeling approach, which uniquely labels heteroconjugates. We have developed an ...[more]