Dam1 complex-microtubule interaction

ABSTRACT: we used cross-linking mass spectrometry to determine the molecular architecture of the Dam1 complex alone and bound to microtubules. Our data provide a map of the subunit arrangement of the Dam1 complex. Analysis of the Dam1 complex assembled around microtubules reveals that the Spc34 and Ask1 subunits are likely involved in self -assembly whereas the Duo1 and Dam1 subunits both interact with microtubules. We also demonstrate that the C termini of Dam1 and Duo1 provide the microtubule binding properties to the Dam1 complex. Our data provides key information on the organization of the Dam1 complex around microtubules.

INSTRUMENT(S):

ORGANISM(S): Candida Albicans (yeast)

SUBMITTER: Juan Zou  

LAB HEAD: Prof. Juri Rappsilber

PROVIDER: PXD003678 | Pride | 2016-03-01

REPOSITORIES: Pride