Name: Human Arginine Methylome - Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells
Keywords: Human hek293 cells

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Human Arginine Methylome - Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells

ABSTRACT: Identification of arginine methylation sites in human HEK293 cells, yielding a total of >8.000 high-confident modification sites on >3.300 proteins

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Electrically Active Cell

DISEASE(S): Disease Free

SUBMITTER: Michael L. Nielsen

LAB HEAD: Michael Lund Nielsen

PROVIDER: PXD003700 | Pride | 2016-09-30

REPOSITORIES: Pride

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Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells.

Larsen Sara C SC Sylvestersen Kathrine B KB Mund Andreas A Lyon David D Mullari Meeli M Madsen Maria V MV Daniel Jeremy A JA Jensen Lars J LJ Nielsen Michael L ML

Science signaling 20160830 443

The posttranslational modification of proteins by arginine methylation is functionally important, yet the breadth of this modification is not well characterized. Using high-resolution mass spectrometry, we identified 8030 arginine methylation sites within 3300 human proteins in human embryonic kidney 293 cells, indicating that the occurrence of this modification is comparable to phosphorylation and ubiquitylation. A site-level conservation analysis revealed that arginine methylation sites are le ...[more]

PMID: 27577262

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Human Arginine Methylome

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