Urea artifacts interfere with immunopurification of lysine acetylation

ABSTRACT: Urea-based buffers can induce carbamylation artefacts. Here we report that carbamylation of lysine residues resembles the structure of acetylated lysines (K-ac) and compete during K-ac immunoaffinity purification. We propose an alternative strategy using an ionic detergent which improves significantly the selectivity and efficiency of K-ac affinity immuno-purifications.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ana Martinez  

LAB HEAD: Javier Muñoz Peralta

PROVIDER: PXD003701 | Pride | 2017-01-11

REPOSITORIES: Pride