Urea artifacts interfere with immunopurification of lysine acetylation
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Bruker Daltonics maXis series
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: 
Ana Martinez
LAB HEAD: Javier Muñoz Peralta
PROVIDER: PXD003701 | Pride | 2017-01-11
REPOSITORIES: Pride
Publications
Urea Artifacts Interfere with Immuno-Purification of Lysine Acetylation.
Martinez-Val Ana A Garcia Fernando F Ximénez-Embún Pilar P Martínez Teresa-Calleja Ailyn A Ibarz Nuria N Ruppen Isabel I Munoz Javier J
Journal of proteome research 20170117 2
Comprehensive analysis of post-translational modifications (PTMs) often depends on the purification of modified peptides prior to LC-MS/MS. The implementation of these enrichment methods requires thorough knowledge of the experimental conditions to achieve optimal selectivity and sensitivity. In this regard, large-scale analysis of lysine acetylation, a key PTM for multiple cellular processes, makes use of monoclonal pan-antibodies designed against this moiety. We report that the immuno-purifica ...[more]
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