Proteomics

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Proteomics analysis of soybean root exposed to varying sizes of silver nanoparticles under flooding stress


ABSTRACT: Because of the tremendous use of NPs in the agricultural sector (Vernikov et al., 2009) and their interactions with the plants, the effect of NPs in plants needs to be explored at the molecular level. To investigate the effects of varying sizes of silver NPs on early-stage soybean under flooding stress, morphological and proteomic analyses were performed. In this study, 5 ppm silver NPs were used because 5 ppm of silver NPs were reported to promote the soybean growth under flooding stress (Mustafa et al., 2015). In the present study, the silver NPs induced changes, in the soybean proteins under flooding stress, was evaluated using gel-free proteomic technique.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Glycine Max

TISSUE(S): Plant Cell, Root

DISEASE(S): Wounds And Injuries

SUBMITTER: Ghazala Mustafa  

LAB HEAD: Setsuko Komatsu

PROVIDER: PXD003711 | Pride | 2016-08-08

REPOSITORIES: Pride

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Publications

Proteomic analysis of soybean root exposed to varying sizes of silver nanoparticles under flooding stress.

Mustafa Ghazala G   Sakata Katsumi K   Komatsu Setsuko S  

Journal of proteomics 20160726


<h4>Unlabelled</h4>Silver nanoparticles (Ag-NPs) are excessively used as antibacterial agents; however, environmental interaction specifically with the plants remain uncertain. To study the size-dependent effects of Ag-NPs on soybean under flooding, a proteomic technique was used. Morphological analysis revealed that treatment with Ag-NPs of 15nm promoted soybean growth under flooding compared to 2 and 50-80nm. A total of 228 common proteins that significantly changed in abundance under flooding  ...[more]

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