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Co-immunoprecipitation of HA-tagged proteins in Giardia lamblia

ABSTRACT: Giardia lamblia's diverged endocytic system was investigated using co-immunoprecipitation of known and conserved endocytic factors which led to the discovery of novel associated components. This was assembled into an interactome of the endocytic pathway in this intestinal parasite.

INSTRUMENT(S): Synapt MS, Q Exactive

ORGANISM(S): Giardia Lamblia Atcc 50803

TISSUE(S): Eukaryotic Cell

DISEASE(S): Giardiasis

SUBMITTER: Carmen Faso

LAB HEAD: Adrian B. Hehl

PROVIDER: PXD003718 | Pride | 2016-06-23

REPOSITORIES: Pride

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Static Clathrin Assemblies at the Peripheral Vacuole-Plasma Membrane Interface of the Parasitic Protozoan Giardia lamblia.

Zumthor Jon Paulin JP Cernikova Lenka L Rout Samuel S Kaech Andres A Faso Carmen C Hehl Adrian B AB

PLoS pathogens 20160720 7

Giardia lamblia is a parasitic protozoan that infects a wide range of vertebrate hosts including humans. Trophozoites are non-invasive but associate tightly with the enterocyte surface of the small intestine. This narrow ecological specialization entailed extensive morphological and functional adaptations during host-parasite co-evolution, including a distinctly polarized array of endocytic organelles termed peripheral vacuoles (PVs), which are confined to the dorsal cortical region exposed to t ...[more]

PMID: 27438602

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