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Populus Palmitoylome - Proteomic Analysis of a Poplar Cell Suspension Culture Suggests a Major Role of Protein S-Acylation in Diverse Cellular Processes

ABSTRACT: The aim of this work was to identify S-acylated proteins in poplar. In order to achieve this, we have combined the acyl-biotin exchange method with a mass spectrometry based proteomic approach. To date, this is the only study on S-acylated proteins in a tree species. In total, we identified around 450 S-acylated proteins, the majority of which are involved in signal transduction, response to stress and transport.

INSTRUMENT(S):

ORGANISM(S): Populus Trichocarpa (western Balsam Poplar) (populus Balsamifera Subsp. Trichocarpa)

TISSUE(S): Plant Cell, Cell Suspension Culture

SUBMITTER: Vaibhav Srivastava

LAB HEAD: Vincent Bulone

PROVIDER: PXD003735 | Pride | 2016-05-09

REPOSITORIES: Pride

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			Action	DRS
	BR1-Hyd_1Populus.zip	Other
	BR1Hyd_1Populus.zip	Other
	BR2-Hyd_2Populus.zip	Other
	BR2Hyd_2Populus.zip	Other
	BR3-Hyd_3Populus.zip	Other

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Publications

Proteomic Analysis of a Poplar Cell Suspension Culture Suggests a Major Role of Protein S-Acylation in Diverse Cellular Processes.

Srivastava Vaibhav V Weber Joseph R JR Malm Erik E Fouke Bruce W BW Bulone Vincent V

Frontiers in plant science 20160412

S-acylation is a reversible post-translational modification of proteins known to be involved in membrane targeting, subcellular trafficking, and the determination of a great variety of functional properties of proteins. The aim of this work was to identify S-acylated proteins in poplar. The use of an acyl-biotin exchange method and mass spectrometry allowed the identification of around 450 S-acylated proteins, which were subdivided into three major groups of proteins involved in transport, signa ...[more]

PMID: 27148305

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