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The dynamic interactome of Polycomb family complexes during stem cell differentiation

ABSTRACT: While the core subunits of Polycomb group (PcG) complexes are well characterized, little is known about the dynamics of these protein complexes during cellular differentiation. We used quantitative interaction proteomics to study PcG proteins in mouse embryonic stem cells (mESCs) and neural progenitor cells (NPCs). We found the stoichiometry of PRC1 and PRC2 to be highly dynamic during neural differentiation.

OTHER RELATED OMICS DATASETS IN: PRJNA299693

INSTRUMENT(S): LTQ FT, LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Multipotent Stem Cell, Cell Culture, Embryonic Stem Cell

SUBMITTER: Susan Kloet

LAB HEAD: Michiel Vermeulen

PROVIDER: PXD003758 | Pride | 2016-05-19

REPOSITORIES: Pride

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The dynamic interactome and genomic targets of Polycomb complexes during stem-cell differentiation.

Kloet Susan L SL Makowski Matthew M MM Baymaz H Irem HI van Voorthuijsen Lisa L Karemaker Ino D ID Santanach Alexandra A Jansen Pascal W T C PWTC Di Croce Luciano L Vermeulen Michiel M

Nature structural & molecular biology 20160613 7

Although the core subunits of Polycomb group (PcG) complexes are well characterized, little is known about the dynamics of these protein complexes during cellular differentiation. We used quantitative interaction proteomics and genome-wide profiling to study PcG proteins in mouse embryonic stem cells (ESCs) and neural progenitor cells (NPCs). We found that the stoichiometry and genome-wide binding of PRC1 and PRC2 were highly dynamic during neural differentiation. Intriguingly, we observed a dow ...[more]

PMID: 27294783

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