Proteomics

Dataset Information

128

The dynamic interactome of Polycomb family complexes during stem cell differentiation


ABSTRACT: While the core subunits of Polycomb group (PcG) complexes are well characterized, little is known about the dynamics of these protein complexes during cellular differentiation. We used quantitative interaction proteomics to study PcG proteins in mouse embryonic stem cells (mESCs) and neural progenitor cells (NPCs). We found the stoichiometry of PRC1 and PRC2 to be highly dynamic during neural differentiation.

INSTRUMENT(S): LTQ FT, LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Mus musculus  

TISSUE(S): Cell Culture

DISEASE(S): Not Available

SUBMITTER: Susan Kloet  

LAB HEAD: Michiel Vermeulen

PROVIDER: PXD003758 | Pride | 2016-05-19

REPOSITORIES: Pride

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Publications

The dynamic interactome and genomic targets of Polycomb complexes during stem-cell differentiation.

Kloet Susan L SL   Makowski Matthew M MM   Baymaz H Irem HI   van Voorthuijsen Lisa L   Karemaker Ino D ID   Santanach Alexandra A   Jansen Pascal W T C PWTC   Di Croce Luciano L   Vermeulen Michiel M  

Nature structural & molecular biology 20160613 7


Although the core subunits of Polycomb group (PcG) complexes are well characterized, little is known about the dynamics of these protein complexes during cellular differentiation. We used quantitative interaction proteomics and genome-wide profiling to study PcG proteins in mouse embryonic stem cells (ESCs) and neural progenitor cells (NPCs). We found that the stoichiometry and genome-wide binding of PRC1 and PRC2 were highly dynamic during neural differentiation. Intriguingly, we observed a dow  ...[more]

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