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Integrative genome annotation of Malassezia sympodialis

ABSTRACT: The skin commensal yeast Malassezia is associated with several skin disorders. To establish a reference resource, we sought to determine the complete genome sequence of Malassezia sympodialis and identify its protein-coding genes. A novel genome annotation workflow combining RNA sequencing, proteomics, and manual curation was developed to determine gene structures with high accuracy.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Malassezia Sympodialis (strain Atcc 42132) (atopic Eczema-associated Yeast)

DISEASE(S): Atopic Dermatitis

SUBMITTER: Yafeng Zhu

LAB HEAD: Janne Lehtiö

PROVIDER: PXD003773 | Pride | 2016-12-20

REPOSITORIES: Pride

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Proteogenomics produces comprehensive and highly accurate protein-coding gene annotation in a complete genome assembly of Malassezia sympodialis.

Zhu Yafeng Y Engström Pär G PG Tellgren-Roth Christian C Baudo Charles D CD Kennell John C JC Sun Sheng S Billmyre R Blake RB Schröder Markus S MS Andersson Anna A Holm Tina T Sigurgeirsson Benjamin B Wu Guangxi G Sankaranarayanan Sundar Ram SR Siddharthan Rahul R Sanyal Kaustuv K Lundeberg Joakim J Nystedt Björn B Boekhout Teun T Dawson Thomas L TL Heitman Joseph J Scheynius Annika A Lehtiö Janne J

Nucleic acids research 20170301 5

Complete and accurate genome assembly and annotation is a crucial foundation for comparative and functional genomics. Despite this, few complete eukaryotic genomes are available, and genome annotation remains a major challenge. Here, we present a complete genome assembly of the skin commensal yeast Malassezia sympodialis and demonstrate how proteogenomics can substantially improve gene annotation. Through long-read DNA sequencing, we obtained a gap-free genome assembly for M. sympodialis (ATCC 4 ...[more]

PMID: 28100699

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