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Drosophila HP1a IP-MS - The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic

ABSTRACT: Heterochromatin is enriched for characteristic epigenetic factors, including Heterochromatin Protein 1a (HP1a) and is essential for many organismal functions. However, how this nuclear domain is organized and functions is unclear. We extensively investigated heterochromatin protein compostion by biochemically purifying Drosophila melanogaster HP1a interactors.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture

SUBMITTER: Joel Swenson

LAB HEAD: Gary H Karpen

PROVIDER: PXD003780 | Pride | 2016-08-18

REPOSITORIES: Pride

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The composition and organization of Drosophila heterochromatin are heterogeneous and dynamic.

Swenson Joel M JM Colmenares Serafin U SU Strom Amy R AR Costes Sylvain V SV Karpen Gary H GH

eLife 20160811

Heterochromatin is enriched for specific epigenetic factors including Heterochromatin Protein 1a (HP1a), and is essential for many organismal functions. To elucidate heterochromatin organization and regulation, we purified Drosophila melanogaster HP1a interactors, and performed a genome-wide RNAi screen to identify genes that impact HP1a levels or localization. The majority of the over four hundred putative HP1a interactors and regulators identified were previously unknown. We found that 13 of 1 ...[more]

PMID: 27514026

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