Proteomics

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PRICKLE1

ABSTRACT: Identification of the protein associated to PRICKLE1. Components of the evolutionarily conserved developmental planar cell polarity (PCP) pathway were recently described to play a prominent role in cancer cell dissemination. However, the molecular mechanisms by which PCP molecules drive the spread of cancer cells remain largely unknown. PRICKLE1 encodes a PCP protein bound to the promigratory serine/threonine kinase MINK1. We identify RICTOR, a member of the mTORC2 complex, as a PRICKLE1-binding partner and show that the integrity of the PRICKLE1-MINK1-RICTOR complex is required for activation of AKT, regulation of focal adhesions and cancer cell migration. Disruption of the PRICKLE1-RICTOR interaction results in a strong impairment of breast cancer cell dissemination in xenograft assays. Finally, we show that up-regulation of PRICKLE1 in basal breast cancers, a subtype characterized by high metastatic potential, is associated with poor metastasis-free survival.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Avais Daulat  

LAB HEAD: Avais M Daulat

PROVIDER: PXD003784 | Pride | 2022-03-02

REPOSITORIES: Pride

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Publications

ECT2 associated to PRICKLE1 are poor-prognosis markers in triple-negative breast cancer.

Daulat Avais M AM   Finetti Pascal P   Revinski Diego D   Silveira Wagner Mônica M   Camoin Luc L   Audebert Stéphane S   Birnbaum Daniel D   Kodjabachian Laurent L   Borg Jean-Paul JP   Bertucci François F  

British journal of cancer 20190411 9

<h4>Background</h4>Triple-negative breast cancers (TNBC) are poor-prognosis tumours candidate to chemotherapy as only systemic treatment. We previously found that PRICKLE1, a prometastatic protein involved in planar cell polarity, is upregulated in TNBC. We investigated the protein complex associated with PRICKLE1 in TNBC to identify proteins possibly involved in metastatic dissemination, which might provide new prognostic and/or therapeutic targets.<h4>Methods</h4>We used a proteomic approach t  ...[more]

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