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Studying the core circuitry of pluripotency by selective isolation of chromatin-associated proteins

ABSTRACT: Here we have developed a method to identify chromatin-bound partners of a protein of interest by selective isolation of chromatin-associated proteins (SICAP) followed by mass spectrometry. We applied SICAP to identify chromatin-binding proteins associated to Oct4, Sox2 and Nanog in mouse embryonic stem (ES) cells.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Mahmoud Reza Rafiee

LAB HEAD: Prof. Dr. Jeroen Krijgsveld

PROVIDER: PXD003798 | Pride | 2016-12-19

REPOSITORIES: Pride

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Expanding the Circuitry of Pluripotency by Selective Isolation of Chromatin-Associated Proteins.

Rafiee Mahmoud-Reza MR Girardot Charles C Sigismondo Gianluca G Krijgsveld Jeroen J

Molecular cell 20161020 3

Maintenance of pluripotency is regulated by a network of transcription factors coordinated by Oct4, Sox2, and Nanog (OSN), yet a systematic investigation of the composition and dynamics of the OSN protein network specifically on chromatin is still missing. Here we have developed a method combining ChIP with selective isolation of chromatin-associated proteins (SICAP) followed by mass spectrometry to identify chromatin-bound partners of a protein of interest. ChIP-SICAP in mouse embryonic stem ce ...[more]

PMID: 27773674

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